EC Number   |
Reference |
|---|
   3.4.22.35 | - |
683417 |
| 3.4.22.35 | 2 mM SDS is the only surfactant that is effective at promoting the refolding and activation of the enzyme |
731356 |
| 3.4.22.35 | dialysis against refolding buffer |
683468 |
| 3.4.22.35 | recombinant His-tagged enzyme, solvation in 20 mM sodium phosphate, pH 7.4, 0.5 M NaCl, 10 mM imidazole, and 8 M urea, refolding and processing of the inactive precursor, ppEhCP112, to a 35.5 kDa mature and active enzyme, EhCP112 |
701062 |
| 3.4.22.35 | renaturation of purified inactive pro-enzyme by a 100fold dilution in a buffer containing reduced and oxidized thiols leading to a soluble inactive pro-enzyme |
653742 |
| 3.4.22.35 | using 50 mM Tris pH 6.8,150 mM NaCl, 5% (v/v) glycerol, 0.5 mM oxidised glutathione, 3 mM reduced glutathione, at 4°C |
732864 |
