EC Number   |
Reference |
|---|
   3.4.21.4 | denatured trypsinogen is rapidly diluted with re-folding buffer (0.7 M guanidine-HCl, 0.1 M Tris-HCl, pH 8.0, containing 10 mM benzamidine, 2 mM L-cysteine, and 1 mM L-cystine) |
700478 |
| 3.4.21.4 | reversible denaturation at pH 12.0 and at pH 2.0 |
95441 |
| 3.4.21.4 | the denatured trypsin is diluted with 1 M diethanolamine or other solutions to a final concentration of 100 nM at pH 8.2, in the case of fully reduced trypsin, the refolding solution also contains 3 mM glutathione and 1 mM oxidized glutathione, the addition of 1.5 M urea resulted in even less recovered activity despite counteracting the formation of precipitates, refolding from fully reduced trypsin is achieved with a substantial approximate 86% recovery of protein yield |
699046 |
