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EC Number Renatured (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.12recombinant wild-type and mutant enzymes, purification involves denaturation and renaturation steps due to the poor solubility of the full-length enzyme 670301
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.12RNase E is renatured on the Ni-NTA resin by decreasing the concentration of urea to 0.8 M 694243
Display the word mapDisplay the reaction diagram Show all sequences 3.1.26.12the diluted Rne is dialyzed against two changes of 100 volumes each of 60 mM NH4Cl, 0.1 mM dithiothreitol, and 10% (v/v) glycerol at 4°C for at least 20 h 694008
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