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EC Number	Renatured (Commentary)	Reference
3.1.13.1	-	134061, 134063
3.1.13.1	recombinant RC-RNase 2 from Escherichia coli strain BL21(DE3) inclusion bodies by solution in 6 M GdnHCl, followed by rapid dilution to a final concentration of 0.07 mg/ml and incubated at 10°C for 24 h with refolding buffer containing Tris-acetate pH 8.5, 0.5 M L-arginine, 3 mM reduced glutathione, and 0.6 mM oxidized glutathione. The refolding solution is concentrated and dialyzed against sodium acetate (pH 5.0)	749739

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Release 2023.1 (January 2023)