3.1.13.1 | recombinant RC-RNase 2 from Escherichia coli strain BL21(DE3) inclusion bodies by solution in 6 M GdnHCl, followed by rapid dilution to a final concentration of 0.07 mg/ml and incubated at 10°C for 24 h with refolding buffer containing Tris-acetate pH 8.5, 0.5 M L-arginine, 3 mM reduced glutathione, and 0.6 mM oxidized glutathione. The refolding solution is concentrated and dialyzed against sodium acetate (pH 5.0) |
749739 |