Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Renatured (Commentary)

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 2 of 2
download as CSV
download all results as CSV
EC Number Renatured (Commentary) Reference
Show all pathways known for 2.7.1.71Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.71the enzyme is fully unfolded in 4 M urea. Approximately 95% of the enzyme activity can be recovered on dilution of the urea from 4 to 0.36 M. Refolding occurs in at least four kinetic phases, the slowest of which corresponds with the regain of shikimate binding and enzyme activity 641913
Show all pathways known for 2.7.1.71Display the word mapDisplay the reaction diagram Show all sequences 2.7.1.71when the enzyme is unfolded by incubation in 4 M urea, addition of NaCl or Na2SO4 leads to relatively slow refolding of the enzyme. The refolded enzyme can bind shikimate, though more weakly than the native enzyme. The refolded enzyme does not appear to be capable of binding nucleotides, nor does it possess detectable catalytic activity. The refolding process brought about by addition of salt in the presence of 4 M urea is not associated with any change in the fluorescence of the probe 8-anilino-1-naphthalenesulfonic acid 641900
Results 1 - 2 of 2
download as CSV
download all results as CSV