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EC Number	Renatured (Commentary)	Reference
2.1.1.63	renaturation by dilution with buffer from 8 M urea or 6 M guanidine HCl instantly restores the secondary structure of the Ada protein to a state that is almost indistinguishable from the native state. Kinetics of renaturation is fast, at 0°C the restoration is quantitative in less than 30 s. The heat-coagulated protein can be restored to full activity by cycling it through treatment with 8 M urea or 6 M guanidine	485504

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Release 2023.1 (January 2023)