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EC Number	Renatured (Commentary)	Reference
1.5.3.1	production of soluble apoenzyme lacking FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD. Autoflavinylation occurs in a reaction that proceeds via reduced flavin intermediate and requires only apoenzyme and FAD	672027
1.5.3.1	reconstitution of holoprotein from inclusion bodies, suitable conditions for binding of flavoprotein to apoprotein are 0.3 g/l protein concentration, pH 8.5, 4°C, ratio of GSH/GSSH 2	765167

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Release 2023.1 (January 2023)