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EC Number	Renatured (Commentary)	Reference
1.5.1.36	-	720976
1.5.1.36	refolding of recombinant enzyme from Escherichia coli strain BL21(DE3) (pLysS) inclusion bodies, with Triton X100 used in the washing buffer, refolding from solubilization buffer containing 8 M urea, 10 mM DTT, 50 mM Tris-HCl, pH 8.0, per mg pellet at 50°C for 30 min, refolding in a 20fold volume of refolding buffer.containing 50 mM TrisHCl, pH 8.0, 240 mM NaCl, 10 mM KCl, 1 mM Na2EDTA, 1 mM reduced glutathione, 0.1 mM oxidized glutathione, and 0.05 % w/v PEG 4000, followed by ultrafiltration	741794

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Release 2023.1 (January 2023)