1.13.11.55 | recombinant wild-type and mutant enzymes from Escherichia coli strain B21 inclusion bodies, purified by nickel affinity chromatography, are refolded by a four-step dialysis using 20 mM Tris/HCl, 150 mM NaCl, pH 8.14, containing 2 mM reduced glutathione, 0.02 mM oxidized glutathione, 5% glycerol, 0.005% Tween 20, and 0.1 mM ferric citrate with 6 M urea in the first step, 3 M urea in the second step, and 1 M urea in the third step |
725890 |