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EC Number	Renatured (Commentary)	Reference
1.1.1.82	renaturation of the guanidine-HCl denatured enzyme is more rapid with the reduced enzyme and occurs with higher yields, 100%, than with the oxidized enzyme, 60-80%	287389
1.1.1.82	solubilization and refolding of recombinant MDH from Escherichia coli inclusion bodies, the enzyme is dissolved in 6 M guanidine-HCl and gradually refolded to the active enzyme through dilution of the denaturant, conditions: refolding buffer 1.5 L of 0.1 M Tris-HCl, pH 7.5, containing 2 mM EDTA and 0.4 M L-arginine, incubation for 36 h at 4°C	696461

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Release 2023.1 (January 2023)