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EC Number
90% renaturation provided the denatured zymogen is solubilized with urea or guanidinium chloride and given time to refold
refolding of denatured recombinant mature wild-type enzyme from inclusion bodies in 100 mM Na-acetate, pH 4.5, 8 M urea, 10 mM DTT, and 280 mM NaCl, by rapid dilution 1:20 into phosphate buffered saline, pH 7.4, with 10 mM DTT
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