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EC Number
kcat/KM Value [1/mMs
-1
]
kcat/KM Value Maximum [1/mMs
-1
]
Substrate
Commentary
Reference
6.3.4.14
0.00006
-
biotin
pH 7.4, mutant R338A
704465
6.3.4.14
0.0001
-
HCO3-
pH 8.5, mutant R338A
704465
6.3.4.14
0.000594
-
HCO3-
pH 8.5, mutant E296A
704465
6.3.4.14
0.00123
-
biotin
pH 7.4, mutant E296A
704465
6.3.4.14
0.0165
-
biotin
pH 7.4 wild-type
704465
6.3.4.14
0.0272
-
HCO3-
pH 8.5, wild-type
704465
6.3.4.14
26
-
HCO3-
wild type enzyme, with 20 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C
727879
6.3.4.14
49
-
HCO3-
wild type enzyme, with 10 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C
727879
6.3.4.14
71
-
HCO3-
wild type enzyme, with 7.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C
727879
6.3.4.14
162
-
HCO3-
wild type enzyme, with 1.5 mM Mg-ATP, in 100 mM HEPES (pH 8.0), at 30°C
727879
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