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EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Commentary	Reference
6.1.1.21	0.15	-	ATP	mutant enzyme S365N, at pH 7.5 and 25°C	745058
6.1.1.21	0.272	-	ATP	mutant enzyme Y330C, at pH 7.5 and 25°C	745058
6.1.1.21	0.38	-	ATP	mutant enzyme V155G, at pH 7.5 and 25°C	745058
6.1.1.21	3	-	L-histidine	mutant enzyme V155G, at pH 7.5 and 25°C	745058
6.1.1.21	4	-	L-histidine	mutant enzyme Y330C, at pH 7.5 and 25°C	745058
6.1.1.21	40	-	L-histidine	mutant enzyme S365N, at pH 7.5 and 25°C	745058
6.1.1.21	130	-	ATP	wild type enzyme, at pH 7.5 and 25°C	745058
6.1.1.21	500	-	L-histidine	wild type enzyme, at pH 7.5 and 25°C	745058
6.1.1.21	1000	-	tRNAHis	pH 7.2, 37°C, wild-type substrate	716387
6.1.1.21	1231	-	tRNAHis	wild-type Caulobacter crescentus tRNAHis, pH 7.5, 30°C	716383

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Release 2023.1 (January 2023)