EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   4.1.3.32 | 0.005 |
- |
(2R,3S)-dimethylmalate |
mutant enzyme C124A, in 50 mM K+-HEPES (pH 7.5 and 25°C) |
693684 |
| 4.1.3.32 | 0.01 |
- |
(2R,3S)-2-methylisocitrate |
wild type enzyme, in 50 mM K+-HEPES (pH 7.5 and 25°C) |
693684 |
| 4.1.3.32 | 0.01 |
- |
(2S,3R)-2-methylisocitrate |
wild type enzyme, in 50 mM K+-HEPES (pH 7.5 and 25°C) |
693684 |
| 4.1.3.32 | 0.1 |
- |
(2R)-ethylmalate |
wild type enzyme, in 50 mM K+-HEPES (pH 7.5 and 25°C) |
693684 |
| 4.1.3.32 | 0.3 |
- |
(2R)-propyl-(3S)-methylmalate |
wild type enzyme, in 50 mM K+-HEPES (pH 7.5 and 25°C) |
693684 |
| 4.1.3.32 | 1 |
- |
(2R)-methylmalate |
wild type enzyme, in 50 mM K+-HEPES (pH 7.5 and 25°C) |
693684 |
| 4.1.3.32 | 20 |
- |
(2R,3S)-dimethylmalate |
mutant enzyme D59S, in 50 mM K+-HEPES (pH 7.5 and 25°C) |
693684 |
| 4.1.3.32 | 30 |
- |
(2R,3S)-dimethylmalate |
mutant enzyme C124S, in 50 mM K+-HEPES (pH 7.5 and 25°C) |
693684 |
| 4.1.3.32 | 50 |
- |
(2R)-ethyl-(3S)-methylmalate |
wild type enzyme, in 50 mM K+-HEPES (pH 7.5 and 25°C) |
693684 |
| 4.1.3.32 | 60 |
- |
(2R,3S)-dimethylmalate |
mutant enzyme D59A, in 50 mM K+-HEPES (pH 7.5 and 25°C) |
693684 |
