EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    4.1.3.3 | 0.002 |
- |
N-acetyl-D-mannosamine |
mutant enzyme P193Y, at pH 7.0 and 37°C |
749150 |
| 4.1.3.3 | 0.0033 |
- |
N-acetylneuraminate |
mutant Y137F enzyme, pH 7.4, 30°C |
726551 |
| 4.1.3.3 | 0.004 |
- |
N-acetyl-D-mannosamine |
mutant enzyme G210S/Y213G, at pH 7.0 and 37°C |
749150 |
| 4.1.3.3 | 0.0074 |
- |
N-acetyl-D-mannosamine |
cross-linked enzyme aggregates, pH 7.5, 37°C |
714508 |
| 4.1.3.3 | 0.016 |
- |
N-acetyl-D-mannosamine |
wild type enzyme, at pH 7.0 and 37°C |
749150 |
| 4.1.3.3 | 0.026 |
- |
N-acetylneuraminate |
mutant enzyme G211S, at pH 7.0 and 37°C |
749150 |
| 4.1.3.3 | 0.03 |
- |
N-acetyl-D-mannosamine |
pH 7.0, 37°C |
713843 |
| 4.1.3.3 | 0.03 |
- |
N-acetyl-D-mannosamine |
recombinant His-taggged enzyme, pH 7.0, 37°C |
727656 |
| 4.1.3.3 | 0.03 |
- |
N-acetyl-D-mannosamine |
soluble enzyme, pH not specified in the publication, temperature not specified in the publication |
714508 |
| 4.1.3.3 | 0.06 |
- |
N-acetylneuraminic acid |
E192V mutant protein, pH 7.5, 25°C |
715921 |
