EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
4.1.3.17 | 0.0019 |
- |
4-hydroxy-2-oxopentanoate |
wild type protein, 1.0 mM Mg2+, pH 8.0, 25°C |
715556 |
4.1.3.17 | 0.00645 |
- |
4-hydroxy-4-methyl-2-oxoglutarate |
mutant enzyme K147A, in the presence of 2 mM phosphate, at pH 8.0 and 25°C |
749095 |
4.1.3.17 | 0.00697 |
- |
4-hydroxy-4-methyl-2-oxoglutarate |
mutant enzyme K147A, in the absence of phosphate, at pH 8.0 and 25°C |
749095 |
4.1.3.17 | 0.0165 |
- |
4-hydroxy-4-methyl-2-oxoglutarate |
mutant enzyme N71A, in the presence of 2 mM phosphate, at pH 8.0 and 25°C |
749095 |
4.1.3.17 | 0.0176 |
- |
4-hydroxy-4-methyl-2-oxoglutarate |
mutant enzyme N71A, in the absence of phosphate, at pH 8.0 and 25°C |
749095 |
4.1.3.17 | 0.0176 |
- |
4-hydroxy-4-methyl-2-oxoglutarate |
mutant enzyme R123K, in the presence of 2 mM phosphate, at pH 8.0 and 25°C |
749095 |
4.1.3.17 | 0.022 |
- |
4-hydroxy-2-oxopentanoate |
wild type protein, 1.0 mM Mn2+, pH 8.0, 25°C |
715556 |
4.1.3.17 | 0.0267 |
- |
4-hydroxy-4-methyl-2-oxoglutarate |
mutant enzyme R123K, in the absence of phosphate, at pH 8.0 and 25°C |
749095 |
4.1.3.17 | 0.0577 |
- |
4-hydroxy-4-methyl-2-oxoglutarate |
mutant enzyme G144V, in the presence of 2 mM phosphate, at pH 8.0 and 25°C |
749095 |
4.1.3.17 | 0.155 |
- |
4-hydroxy-4-methyl-2-oxoglutarate |
mutant enzyme G144V, in the absence of phosphate, at pH 8.0 and 25°C |
749095 |