EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    4.1.1.7 | 0.012 |
- |
pyruvate |
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C |
702341 |
| 4.1.1.7 | 0.065 |
- |
pyruvate |
mutant enzyme A460Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C |
702341 |
| 4.1.1.7 | 0.1 |
- |
phenylpyruvate |
wild type enzyme, at pH 6.0 and 30°C |
747471 |
| 4.1.1.7 | 0.22 |
- |
benzoylformate |
wild type enzyme, at pH 6.0 and 30°C |
747171 |
| 4.1.1.7 | 0.3 |
- |
benzoylformate |
mutant enzyme I466A, at pH 6.0 and 30°C |
747171 |
| 4.1.1.7 | 0.3 |
- |
phenylglyoxylate |
pH and temperature not specified in the publication, mutant H70A |
727483 |
| 4.1.1.7 | 0.42 |
- |
pyruvate |
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C |
702341 |
| 4.1.1.7 | 0.5 |
- |
2-ketobutanoate |
wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C |
702341 |
| 4.1.1.7 | 0.5 |
- |
2-oxobutanoate |
wild type enzyme, at pH 6.0 and 30°C |
747471 |
| 4.1.1.7 | 0.86 |
- |
2-ketohexanoate |
mutant enzyme T377L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C |
702341 |
