EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.4.24.27 | 0.23 |
- |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
mutant enzyme S53D/L155A/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C |
712532 |
3.4.24.27 | 0.26 |
- |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
mutant enzyme L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C |
712532 |
3.4.24.27 | 0.29 |
- |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
mutant enzyme G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C |
712532 |
3.4.24.27 | 0.29 |
- |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
wild type enzyme, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C |
712532 |
3.4.24.27 | 0.31 |
- |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
mutant enzyme S53D/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C |
712532 |
3.4.24.27 | 0.32 |
- |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
mutant enzyme S53D, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C |
712532 |
3.4.24.27 | 0.33 |
- |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
mutant enzyme S53D/L155A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C |
712532 |
3.4.24.27 | 0.34 |
- |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
mutant enzyme L155A/G8C/N60C/S65P, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C |
712532 |
3.4.24.27 | 0.62 |
- |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
mutant enzyme I168A, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C |
712532 |
3.4.24.27 | 0.79 |
- |
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide |
mutant enzyme D150E, in the absence of 4 M NaCl, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C |
712532 |