EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.4.22.B50 | 0.01 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant Y265A, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 0.045 |
- |
RLRGG-7-amido-4-methylcoumarin |
wild type enzyme, at pH 8.0 and 37°C |
732827 |
3.4.22.B50 | 0.055 |
- |
Dabcyl-FRLKGGAPIKGV-Edans |
mutant T39W, pH 6.5, 30°C |
752517 |
3.4.22.B50 | 0.058 |
- |
Dabcyl-FRLKGGAPIKGV-Edans |
wild-type, pH 6.5, 30°C |
752517 |
3.4.22.B50 | 0.065 |
- |
RLRGG-7-amido-4-methylcoumarin |
mutant enzyme I353R, at pH 8.0 and 37°C |
732827 |
3.4.22.B50 | 0.08 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant D165A, pH 7.4, 30°C |
731069 |
3.4.22.B50 | 0.082 |
- |
Dabcyl-FKKKGGGDVKE-Edans |
wild-type, pH 6.5, 30°C |
752517 |
3.4.22.B50 | 0.103 |
- |
Dabcyl-FKKKGGGDVKE-Edans |
mutant T39W, pH 6.5, 30°C |
752517 |
3.4.22.B50 | 0.155 |
- |
RLRGG-7-amido-4-methylcoumarin |
mutant enzyme I353W, at pH 8.0 and 37°C |
732827 |
3.4.22.B50 | 0.23 |
- |
ubiquitin-7-amido-4-trifluoromethylcoumarin |
recombinant mutant E168A, pH 7.4, 30°C |
731069 |