EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   3.4.22.59 | 0.15 |
- |
Ac-Val-Glu-Ile-Asp-7-amido-4-trifluoromethylcoumarin |
37°C, pH 7.2, mutant enzyme L200A |
755524 |
| 3.4.22.59 | 1.39 |
- |
receptor-interacting protein kinase-1 |
in 0.8 M sodium citrate, 20 mM PIPES, 100 mM NaCl, pH 7.2, 5 mM dithiothreitol, at 37°C |
731602 |
| 3.4.22.59 | 2.53 |
- |
N-acetyl-Ile-Val-Leu-Asp-7-amido-4-methylcoumarin |
- |
707434 |
| 3.4.22.59 | 37 |
- |
N-acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin |
- |
707434 |
| 3.4.22.59 | 44 |
- |
N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin |
- |
707434 |
| 3.4.22.59 | 46.833 |
- |
Ac-Val-Glu-Ile-Asp-7-amido-4-trifluoromethylcoumarin |
37°C, pH 7.2, mutant enzyme E35K |
755524 |
| 3.4.22.59 | 51.9 |
- |
N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin |
pH 7.5, 37°C, mutant enzyme R44K |
754186 |
| 3.4.22.59 | 54.7 |
- |
N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin |
pH 7.5, 37°C, mutant enzyme R42A/R43A/R44A |
754186 |
| 3.4.22.59 | 58.3 |
- |
N-acetyl-Val-Glu-Ile-Asp-7-amido-4-methylcoumarin |
pH 7.5, 37°, wild-type enzyme |
754186 |
| 3.4.22.59 | 58.65 |
- |
Ac-Val-Glu-Ile-Asp-7-amido-4-trifluoromethylcoumarin |
37°C, pH 7.2, mutant enzyme K284A |
755524 |
