EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   3.2.2.27 | -999 |
- |
more |
higher catalytic efficiency (kcat/Km) towards single-stranded DNA due to the smaller Km value |
721666 |
| 3.2.2.27 | 0.00065 |
- |
uracil-containing calf thymus DNA |
mutant enzyme D183G, at pH 7.5 and 15°C |
750352 |
| 3.2.2.27 | 0.0007 |
- |
uracil-containing calf thymus DNA |
mutant enzyme D183G/K302R, at pH 7.5 and 15°C |
750352 |
| 3.2.2.27 | 0.0011 |
- |
uracil-containing calf thymus DNA |
wild type enzyme, at pH 7.5 and 15°C |
750352 |
| 3.2.2.27 | 0.00147 |
- |
uracil-containing calf thymus DNA |
mutant enzyme D183G/K302R, at pH 7.5 and 22°C |
750352 |
| 3.2.2.27 | 0.00163 |
- |
uracil-containing calf thymus DNA |
mutant enzyme D183G, at pH 7.5 and 22°C |
750352 |
| 3.2.2.27 | 0.00178 |
- |
uracil-containing calf thymus DNA |
mutant enzyme G183D, at pH 7.5 and 15°C |
750352 |
| 3.2.2.27 | 0.00225 |
- |
uracil-containing calf thymus DNA |
wild type enzyme, at pH 7.5 and 22°C |
750352 |
| 3.2.2.27 | 0.0032 |
- |
uracil-containing calf thymus DNA |
mutant enzyme G183D/R302K, at pH 7.5 and 15°C |
750352 |
| 3.2.2.27 | 0.0052 |
- |
uracil-containing calf thymus DNA |
wild type enzyme, at pH 7.5 and 37°C |
750352 |
