EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    3.1.1.23 | 1.5 |
- |
umbelliferyl arachidonate |
wild type enzyme, in 20 mM PIPES, pH 7.0, and 150 mM NaCl at 37°C |
716864 |
| 3.1.1.23 | 1.7 |
- |
umbelliferyl arachidonate |
mutant enzyme L169S/L176S, in 20 mM PIPES, pH 7.0, and 150 mM NaCl at 37°C |
716864 |
| 3.1.1.23 | 2.1 |
- |
arachidonoyl-1-thio-glycerol |
in 10 mM Tris-HCl (pH 7.2), temperature not specified in the publication |
730289 |
| 3.1.1.23 | 3.3 |
- |
4-Methylumbelliferyl butyrate |
mutant enzyme L169S/L176S/K165A, in 20 mM PIPES, pH 7.0, and 150 mM NaCl at 37°C |
716864 |
| 3.1.1.23 | 4.2 |
- |
4-Methylumbelliferyl butyrate |
mutant enzyme L169S/L176S/K36A/K226A, in 20 mM PIPES, pH 7.0, and 150 mM NaCl at 37°C |
716864 |
| 3.1.1.23 | 5.5 |
- |
4-Methylumbelliferyl butyrate |
mutant enzyme L169S/L176S/K160A, in 20 mM PIPES, pH 7.0, and 150 mM NaCl at 37°C |
716864 |
| 3.1.1.23 | 5.8 |
- |
4-Methylumbelliferyl butyrate |
mutant enzyme L169S/L176S, in 20 mM PIPES, pH 7.0, and 150 mM NaCl at 37°C |
716864 |
| 3.1.1.23 | 5.8 |
- |
4-Methylumbelliferyl butyrate |
mutant enzyme L169S/L176S/K226A, in 20 mM PIPES, pH 7.0, and 150 mM NaCl at 37°C |
716864 |
| 3.1.1.23 | 6.8 |
- |
4-Methylumbelliferyl butyrate |
mutant enzyme L169S/L176S/K36A, in 20 mM PIPES, pH 7.0, and 150 mM NaCl at 37°C |
716864 |
| 3.1.1.23 | 7 |
- |
4-Methylumbelliferyl butyrate |
wild type enzyme, in 20 mM PIPES, pH 7.0, and 150 mM NaCl at 37°C |
716864 |
