EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   2.7.1.23 | 0.19 |
- |
polyphosphate(65) |
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C |
721366 |
| 2.7.1.23 | 0.24 |
- |
ATP |
wild type enzyme, at pH 7.5 and 30°C |
722144 |
| 2.7.1.23 | 0.24 |
- |
polyphosphate(45) |
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C |
721366 |
| 2.7.1.23 | 0.36 |
- |
NAD+ |
mutant enzyme P57S/P117S, at pH 6.0 and 30°C |
722144 |
| 2.7.1.23 | 0.39 |
- |
NAD+ |
wild type enzyme, at pH 7.5 and 30°C |
722144 |
| 2.7.1.23 | 0.42 |
- |
polyphosphate(25) |
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C |
721366 |
| 2.7.1.23 | 0.5 |
- |
ADP |
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C |
721366 |
| 2.7.1.23 | 0.56 |
- |
ATP |
mutant enzyme P57S/P117S, at pH 6.0 and 30°C |
722144 |
| 2.7.1.23 | 0.58 |
- |
polyphosphate(75) |
in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C |
721366 |
| 2.7.1.23 | 0.94 |
- |
NAD+ |
wild type enzyme, at pH 7.5 and 30°C |
722144 |
