EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
2.4.1.133 | 0.13 |
- |
4-nitrophenyl O-beta-D-xylopyranoside |
mutant enzyme R270C, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication |
718777 |
2.4.1.133 | 0.17 |
- |
4-methylumbelliferyl-beta-D-xylopyranoside |
pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli |
719897 |
2.4.1.133 | 0.22 |
- |
4-nitrophenyl O-beta-D-xylopyranoside |
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication |
718777 |
2.4.1.133 | 0.3 |
- |
4-nitrophenyl O-beta-D-xylopyranoside |
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication |
718777 |
2.4.1.133 | 0.35 |
- |
4-methylumbelliferyl O-beta-D-xylopyranoside |
mutant enzyme L206P, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication |
718777 |
2.4.1.133 | 0.43 |
- |
4-methylumbelliferyl O-beta-D-xylopyranoside |
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication |
718777 |
2.4.1.133 | 0.47 |
- |
4-methylumbelliferyl alpha-D-xylopyranoside |
mutant enzyme Y196F, at pH 7.0 and 37°C |
736498 |
2.4.1.133 | 0.47 |
- |
4-methylumbelliferyl-beta-D-xylopyranoside |
pH 7.0, 37°C, recombinant mutant Y196F |
736498 |
2.4.1.133 | 0.48 |
- |
UDP-galactose |
mutant enzyme A186D, in 100 mM cacodylate buffer (pH 7.0) containing 10 mM MnCl2, temperature not specified in the publication |
718777 |
2.4.1.133 | 1.2 |
- |
4-methylumbelliferyl alpha-D-xylopyranoside |
mutant enzyme R270A, at pH 7.0 and 37°C |
736498 |