EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
2.3.3.13 | 0.16 |
- |
4-methylthio-2-oxobutyrate |
isoform IPMS3, pH and temperature not specified in the publication |
737033 |
2.3.3.13 | 2.3 |
- |
2-oxoisovalerate |
isoform IPMS3, pH and temperature not specified in the publication |
737033 |
2.3.3.13 | 7.5 |
- |
2-oxoisovalerate |
isoform IPMS1, pH and temperature not specified in the publication |
737033 |
2.3.3.13 | 22 |
- |
3-methyl-2-oxobutanoate |
pH 8.0, 50°C |
755968 |
2.3.3.13 | 27 |
- |
acetyl-CoA |
wild type enzyme, at pH 7.5 and 25°C |
735592 |
2.3.3.13 | 33 |
- |
acetyl-CoA |
mutant enzyme R97A, at pH 7.5 and 25°C |
735592 |
2.3.3.13 | 70 |
- |
acetyl-CoA |
mutant enzyme D444A, at pH 7.5 and 25°C |
735592 |
2.3.3.13 | 73 |
- |
acetyl-CoA |
pH 8.0, 50°C |
755968 |
2.3.3.13 | 80 |
- |
acetyl-CoA |
mutant enzyme D444Y, at pH 7.5 and 25°C |
735592 |
2.3.3.13 | 210 |
- |
3-methyl-2-oxobutanoate |
mutant enzyme D444Y, at pH 7.5 and 25°C |
735592 |