EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
   2.1.1.258 | 0.02 |
- |
[Co(I) corrinoid Fe-S protein] |
mutant enzyme N199A, in 0.1 M potassium succinate, pH 5.0, 60 mM NaCl, at 25°C |
719846 |
| 2.1.1.258 | 2.1 |
- |
methylcobalamin |
in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
| 2.1.1.258 | 4.4 |
- |
tetrahydrofolate |
using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
| 2.1.1.258 | 5.2 |
- |
tetrahydrofolate |
using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
| 2.1.1.258 | 14.7 |
- |
hydroxocobalamin |
in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
| 2.1.1.258 | 17.1 |
- |
[methyl-Co(III) corrinoid Fe-S protein] |
in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
| 2.1.1.258 | 150 |
- |
Cobalamin |
25°C, pH 5.1 |
728242 |
| 2.1.1.258 | 450 |
- |
5-methyltetrahydrofolate |
using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
| 2.1.1.258 | 500 |
- |
[Co(I) corrinoid Fe-S protein] |
wild type enzyme, in 0.1 M potassium succinate, pH 5.0, 60 mM NaCl, at 25°C |
719846 |
| 2.1.1.258 | 620 |
- |
Cobalamin |
25°C, pH 7.2 |
728242 |
