EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.1.1.357 | -999 |
- |
more |
Kcat/Km-value for the 17beta-oxidation of testosterone is 0.183 1/sec*mM, and Kcat/Km-value for the 17beta-reduction of androstenedione is 0.817 1/sec*mM |
725345 |
1.1.1.357 | 0.00015 |
- |
cholic acid |
recombinant His-tagged mutant S114A/Y153F, pH 8.0, 37°C |
760734 |
1.1.1.357 | 0.00044 |
- |
Dehydrocholic acid |
recombinant His-tagged mutant Y153F, pH 8.0, 37°C |
760734 |
1.1.1.357 | 0.0005 |
- |
cholic acid |
recombinant His-tagged mutant K157A, pH 8.0, 37°C |
760734 |
1.1.1.357 | 0.0013 |
- |
cholic acid |
recombinant His-tagged mutant Y153F, pH 8.0, 37°C |
760734 |
1.1.1.357 | 0.0014 |
- |
cholic acid |
recombinant His-tagged mutant S114A, pH 8.0, 37°C |
760734 |
1.1.1.357 | 0.0037 |
- |
Dehydrocholic acid |
recombinant His-tagged mutant S114A, pH 8.0, 37°C |
760734 |
1.1.1.357 | 0.015 |
- |
Cyclohexanol |
pH 10.5, 40°C |
760867 |
1.1.1.357 | 0.1 |
- |
Cyclohexanol |
reombinant His-tagged enzyme, pH 10.5, 25°C |
742260 |
1.1.1.357 | 0.411 |
- |
cholic acid |
recombinant His-tagged wild-type enzyme, pH 8.0, 37°C |
760734 |