EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    1.1.1.35 | 0.0056 |
- |
NADH |
mutant enzyme H138A, at pH 7.5 and 37°C |
760296 |
| 1.1.1.35 | 0.011 |
- |
3-acetoacetyl-CoA |
mutant enzyme H138A, at pH 7.5 and 37°C |
760296 |
| 1.1.1.35 | 0.27 |
- |
NADH |
wild type enzyme, at pH 7.5 and 37°C |
760296 |
| 1.1.1.35 | 0.63 |
- |
3-acetoacetyl-CoA |
wild type enzyme, at pH 7.5 and 37°C |
760296 |
| 1.1.1.35 | 90 |
- |
(R)-3-hydroxyacyl-CoA |
recombinant mutant R204A/Y209A, pH 7.0, 25°C |
741307 |
| 1.1.1.35 | 260 |
- |
(S)-3-hydroxybutanoyl-CoA |
pH 8.0, 70°C |
726748 |
| 1.1.1.35 | 300 |
- |
(R)-3-hydroxyacyl-CoA |
recombinant mutants R204A and Y209A, pH 7.0, 25°C |
741307 |
| 1.1.1.35 | 600 |
- |
(R)-3-hydroxyacyl-CoA |
recombinant wild-type enzyme, pH 7.0, 25°C |
741307 |
| 1.1.1.35 | 1700 |
- |
3-oxooctanoyl-CoA |
enzyme Had, at pH 8.0 and 30°C |
761558 |
| 1.1.1.35 | 2500 |
- |
3-oxohexanoyl-CoA |
enzyme Had, at pH 8.0 and 30°C |
761558 |
