EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate  |
Reference |
|---|
   2.7.4.25 | 0.083 |
- |
ara-CMP |
mutant enzyme D185A, at 30°C and pH 7.4 |
712736 |
| 2.7.4.25 | 1.7 |
- |
ara-CMP |
mutant enzyme R181M, at 30°C and pH 7.4 |
712736 |
| 2.7.4.25 | 7.5 |
- |
ara-CMP |
mutant enzyme S101A, at 30°C and pH 7.4 |
712736 |
| 2.7.4.25 | 105 |
- |
ara-CMP |
wild type enzyme, at 30°C and pH 7.4 |
712736 |
| 2.7.4.25 | 0.0114 |
- |
CMP |
mutant enzyme R110M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C |
692282 |
| 2.7.4.25 | 0.053 |
- |
CMP |
mutant enzyme D132H, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C |
692282 |
| 2.7.4.25 | 0.12 |
- |
CMP |
mutant enzyme R188M, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C |
692282 |
| 2.7.4.25 | 0.54 |
- |
CMP |
mutant enzyme D132N, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C |
692282 |
| 2.7.4.25 | 0.54 |
- |
CMP |
mutant enzyme D185A, at 30°C and pH 7.4 |
712736 |
| 2.7.4.25 | 1.41 |
- |
CMP |
mutant enzyme D132A, in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30°C |
692282 |
