EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    1.5.1.30 | 0.0012 |
- |
FMN |
coupled oxidoreductase-luciferase assay, in 100 mM Na+/K+ phosphate buffer with 100 mM NaCl, pH 7.0, 1 microM Fre oxidoreductase, 10 microM decanal, 10 microM NADPH, 5 microM luciferase |
698992 |
| 1.5.1.30 | 0.021 |
- |
riboflavin |
coupled oxidoreductase-luciferase assay, in 100 mM Na+/K+ phosphate buffer with 100 mM NaCl, pH 7.0, 1 microM Fre oxidoreductase, 10 microM decanal, 10 microM NADPH, 5 microM luciferase, riboflavin is favoured by Fre oxidoreductase but a poor substrate for bacterial luciferase |
698992 |
| 1.5.1.30 | 25 |
- |
NADPH |
mutant enzyme K167A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C |
724343 |
| 1.5.1.30 | 150 |
- |
NADPH |
mutant enzyme N134A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C |
724343 |
| 1.5.1.30 | 570 |
- |
NADPH |
mutant enzyme R133A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C |
724343 |
| 1.5.1.30 | 12 |
- |
NADPH |
mutant enzyme R15A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C |
724343 |
| 1.5.1.30 | 2000 |
- |
NADPH |
mutant enzyme R225A, in 20 mM N-tris[hydroxymethyl]methyl-3-aminopropanesulfonic acid buffer, pH 7.0, at 22°C |
724343 |
| 1.5.1.30 | 1.9 |
- |
FMN |
pH 7.5, 25°C |
714077 |
| 1.5.1.30 | 2.4 |
- |
FAD |
pH 7.5, 25°C |
714077 |
| 1.5.1.30 | 5.6 |
- |
oxidized riboflavin |
pH 7.5, 25°C |
714077 |
