EC Number   |
General Information |
Reference |
|---|
    6.3.1.5 | evolution |
the genes encoding the enzymes nicotinic acid mononucleotide adenylyltransferase (NaMNAT), NAD synthetase and NAD kinase responsible for the downstream conversion of nicotinic acid mononucleotide (NaMN) to NAD and NAD(P) are conserved in almost all investigated bacterial genomes |
-, 743959 |
| 6.3.1.5 | malfunction |
induced degradation of NadE enzyme shows a strong bactericidal effect which coincided with anticipated changes in relative levels of NaAD intermediate (substrate of NadE) and ultimate depletion of the NAD(H) pool. The disruption of NAD production in the cell via genetic suppression of the essential enzymes (NadD and NadE) involved in the last two steps of NAD biogenesis leads to cell death, even under dormancy conditions. Proteolytic degradation of the enzyme leads to depletion of the NAD cofactor pool, which suppresses metabolic flux through numerous NAD(P)-dependent pathways of central metabolism of carbon and energy production, phenotype, overview |
745674 |
| 6.3.1.5 | malfunction |
the disruption of NAD production in the cell via genetic suppression of the essential enzymes (NadD and NadE) involved in the last two steps of NAD biogenesis leads to cell death, even under dormancy conditions. Proteolytic degradation of either target enzyme leads to depletion of the NAD cofactor pool, which suppresses metabolic flux through numerous NAD(P)-dependent pathways of central metabolism of carbon and energy production |
745674 |
| 6.3.1.5 | metabolism |
last step of NAD synthesis |
-, 728574 |
| 6.3.1.5 | metabolism |
NAD synthetases are critical as they catalyze the amidation of nicotinic acid adenine dinucleotide (NaAD) to yield the enzyme cofactor NAD, which is the last step in NAD synthesis. NAD synthetase catalyzes the last step in the biosynthesis of nicotinamide adenine dinucleotide, making it a crucial intermediate enzyme linked to the biosynthesis of several amino acids, purine and pyrimidine nucleotides, coenzymes and antibiotics |
-, 743959 |
| 6.3.1.5 | metabolism |
NaMN adenylyltransferase (NadD) and NAD synthetase (NadE) are the key enzymes of NAD biosynthesis |
745674 |
| 6.3.1.5 | physiological function |
existence of an alternative route of NAD synthesis where the amidation of NaMN to nicotinamide mononucleotide occurs before the adenylylation reaction, which converts the intermediate to the NAD cofactor. The first step is catalyzed by Francisella tularensis NadE |
706533 |
