EC Number   |
General Information |
Reference |
|---|
    6.2.1.48 | physiological function |
enzyme is part of the caiTABCDE operon necessary for carnitine metabolism |
728312 |
| 6.2.1.48 | physiological function |
enzyme is part of the caiTABCDEF operon. The caiA gene product is a crotonobetainyl-CoA reductase catalysing the irreversible reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. CaiB acts as betainyl-CoA transferase specific for carnitine, crotonobetaine, gamma-butyrobetaine and its CoA derivatives. CaiD is a crotonobetainyl-CoA hydratase, and CaiC is suggested to be a betainyl-CoA ligase |
-, 724139 |
| 6.2.1.48 | physiological function |
overexpression of CaiC in Escherichia coli enhances the biotransformation of crotonobetaine to L-carnitine nearly 20fold, the yield reaching up to 30% (with growing cells). Higher yields are obtained using resting cells (up to 60%), even when D-carnitine is used as substrate |
740589 |
| 6.2.1.48 | physiological function |
the overexpression of CaiB (CoA-transferase) and CaiC (CoA-ligase) leads to an increase in L-carnitine production. Under optimal concentrations of inducer and fumarate (used as electron acceptors), yields reach 10- and 50fold, respectively, that obtained for the wild type strain. Low levels of coenzyme A limits the activity of these two enzymes |
728014 |
