EC Number   |
General Information |
Reference |
|---|
   4.6.1.22 | evolution |
RNase J is a key member of the beta-CASP family of metallo-beta-lactamases |
730975 |
| 4.6.1.22 | evolution |
the enzyme belongs to the beta-CASP subfamily of metallo-beta-lactamases |
730404 |
| 4.6.1.22 | malfunction |
conditional mutants for both RNases J1 and J2 require induction for growth |
730404 |
| 4.6.1.22 | malfunction |
DELTAermC mRNA shows increased stability in a RNase J1 mutant strain, that contains reduced level of RNase J1. Insertion of a strong stem-loop structure at +65 results in increased stability. Weakening this stem-loop structure results in reversion to wild-type stability. RNA fragments containing the 3' end are detected in a strain with reduced RNase J1 expression, but are undetectable in the wild-type. The 5' ends of these fragments map to the upstream side of predicted stem-loop structures, consistent with an impediment to RNase J1 5' exonuclease processivity. RNase J1 is involved even in decay of the RNA encoded by a 129-nt deletion construct, perhaps by a 59-to-39 exonucleolytic pathway or by cleavage at sites in the upstream half of the RNA. Deletion of the three target sites results in increased mRNA half-life (ca. 15 min) |
710551 |
| 4.6.1.22 | malfunction |
depletion of RNase J1 in a strain also lacking RNase J2 leads to a modest increase in global mRNA stability. transcriptome analysis shows a relatively minor effect of depleting cells for RNase J1 alone or inactivating RNase J2. The double mutant has altered levels for over 600 transcripts with about equal numbers of transcripts showing increased and decreased levels, significant increase in half-life for four specific transcripts cspC, spoVG, tagDccand yweA. Depletion of RNase J1 leads to an accumulation of 3' fragments of RNA |
730932 |
| 4.6.1.22 | malfunction |
lowering RNase Y concentration may affect RNA decay indirectly via an effect on RNase J1, which is thought to exist with RNase Y in a degradosome complex |
-, 729973 |
| 4.6.1.22 | malfunction |
mRNAs are upregulated following enzyme depletion, transcript profile of total RNA preparations from duplicate cultures of the depletion strain SSB447, overview |
730720 |
| 4.6.1.22 | malfunction |
rpsO mRNA half-life is unchanged in a strain that has decreased RNase J1 activity and no RNase J2 activity, but it is 2.3fold higher in a strain with decreased activity of RNase Y |
729917 |
| 4.6.1.22 | metabolism |
Bacillus subtilis endonucleases Bs-RNase III, RNase M5, RNase P, RNase Z, EndoA, and Mini-III are not involved in rpsO mRNA decay, the upstream products are degraded by polynucleotide phosphorylase (PNPase), and the downstream products were degraded by the 5' exonuclease activity of RNase J1 |
729917 |
| 4.6.1.22 | more |
modeling of the enzyme reaction involving the binding of the RNA to the surface of the beta-CASP domain to explain the enzyme's processive action, enzyme residues involved in RNA recognition, overview. RNA-binding channel and proposed nucleotide exit tunnel of RNase J, model of Bacillus subtilis RNase J1/J2 heterodimer bound to RNA, overview |
730975 |
