EC Number   |
General Information |
Reference |
|---|
    4.2.1.109 | evolution |
the MtnB domain of Tetrahymena thermophila MtnBD acquired the new function to catalyze both the dehydratase and enolase reactions through evolutionary gene mutations, rather than fusion of MSP genes |
728615 |
| 4.2.1.109 | malfunction |
the enzyme depletion specifically impairs the capacity of cells to grow in media where methionine is replaced by 5-methylthioadenosine. Knockdown of the enzyme specifically affects the recycling of methionine, and mutation of three potential phosphorylation sites does not affect the enzyme activity whereas mutation of the potential zinc binding site completely abrogates it |
728588 |
| 4.2.1.109 | metabolism |
the enzyme catalyzes the dehydratase step in the methionine salvage pathway |
728588 |
| 4.2.1.109 | metabolism |
the enzyme is involved in the methionine salvage pathway |
730835 |
| 4.2.1.109 | metabolism |
the MtnBD fusion enzyme in Tetrahymena thermophila catalyzes two of the sequential reactions from the dehydratase to dioxygenase steps in the methionine salvage pathway: 5-methylthioribulose-1-phosphate is converted to 2-oxo-4-methylthiobutyrate, the methionine precursor, by four steps, namely dehydratase, enolase, phosphatase, and dioxygenase reactions. The MtnB domain catalyzes dehydration and enolization and the MtnD domain catalyzes dioxygenation, the phosphatase is distinct from the enzyme. Methionine salvage pathway, overview |
728615 |
| 4.2.1.109 | more |
active site architecture and catalytic mechanism, overview |
730835 |
| 4.2.1.109 | more |
identification of the domains responsible for catalyzing the four reactions from the enzyme, overview |
728615 |
| 4.2.1.109 | physiological function |
Apaf-1-interacting protein (APIP) is known to inhibit two different types of cell death: caspase-1-dependent pyroptosis and caspase-9-dependent apoptosis. The enzyme is also involved in the methionine-salvage pathway, where it is called 5-methylthioribulose-1-phosphate dehydratase (MtnB). The enzyme activity seems to be essential for inhibition of pyroptosis by the enzyme, but not for inhibition of apoptosis, overview |
728896 |
| 4.2.1.109 | physiological function |
the enzyme Apaf-1 interacting protein/5-methylthioribulose-1-phosphate dehydratase has two distinct functions, cell death inhibition and methionine salvage. It functions as a cell death inhibitor independently of its MtnB enzyme activity for apoptosis, but dependently for caspase-1-induced pyroptosis. Pyroptosis inhibition by the Apaf-1 interacting protein is dependent upon its MtnB enzyme activity. Role of Apaf-1 interacting protein/5-methylthioribulose-1-phosphate dehydratase in development of cancers and inflammatory diseases. The enzyme acts as an inhibitor of caspase-9-dependent apoptosis induced by ischemic/hypoxic injury or by cytotoxic agents such as etoposide and cisplatin |
730835 |
| 4.2.1.109 | physiological function |
the enzyme is involved in the methionine salvage pathway that recycles the sulfur-containing metabolite 5-methylthioadenosine to methionine |
728588 |
