EC Number |
General Information |
Reference |
---|
4.1.99.11 | evolution |
enzyme BSS is a member of the glycyl radical enzyme (GRE) family |
748195, 749165 |
4.1.99.11 | evolution |
enzyme BSS is a member of the glycyl radical enzyme (GRE) family. Structure and function of benzylsuccinate synthase and related fumarate-adding glycyl radical enzymes, phylogenetic analysis, overview |
-, 748432 |
4.1.99.11 | evolution |
the enzyme belongs to the glycyl radical enzyme family |
727687 |
4.1.99.11 | metabolism |
BSSA gene encoding the alpha-subunit of Bss from a novel taxon of the Betaproteobacteria catalyzes the first step in anaerobic toluene degradation |
-, 703741 |
4.1.99.11 | more |
enzyme structure and structure-based mechanistic models, overview. The QM cluster model contains whole or fragmented side chains of nine active site amino acids surrounding the bound substrates, Glu189, Tyr197, Ser199, Ile384, Leu391, Leu492, Cys493, Arg508, and Val709 |
-, 748432 |
4.1.99.11 | more |
structure homology modeling |
747978 |
4.1.99.11 | more |
subunit BSSalpha adopts a conserved Gly radical enzyme fold. The beta subunit BSSbeta adopts a fold similar to that of a high potential iron-sulfur protein HiPIP. Subunit gamma, BSSgamma, is only 19% identical to BSSbeta, but they share the basic HiPIP fold along with the extended beta-sheet and hairpin loop. Analysis of BSSalpha-BSSgamma subunit interactions and of BSSalpha-BSSbeta subunit interactions, global structural changes in the BSSalpha-BSSgamma complex, influence of BSSbeta on the structure and flexibility of BSSalpha, overview |
749165 |
4.1.99.11 | more |
the C-C bond-forming reaction performed by BSS and its relatives requires an oxygen-sensitive radical cofactor. BSS is a member of the glycyl radical enzyme (GRE) family and contains a backbone glycyl radical in its activated form |
748195 |
4.1.99.11 | more |
the catalytic subunit has the putative radical sites located on Cys492 and Gly828, induced-fit product docking approach and substrate-bound molecular dynamics simulations based on the refined active site topology, three-dimensional structure homology modeling, overview |
727687 |
4.1.99.11 | physiological function |
a novel benzylsuccinate synthase alpha-subunit (bssA) sequence type in sedimentary microbial community originating from a tar-oil-contaminated aquifer at a former coal gasification plant |
704111 |