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Results 1 - 9 of 9
EC Number General Information Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.6malfunction ExoI- ExoVII- SbcCD- cells are sensitive to mitomycin C and 2-aminopurine. Cells lacking all three nucleases, exonuclease I, exonuclease VII, or SbcCD, are inviable without RecG. Removing RNase HI mimics the effect of removing RecG in that DrnhA cells are inviable if ExoI, ExoVII, and SbcCD are eliminated -, 715119
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.6malfunction the absence of 3' exonucleases leads to an accumulation of 3' flaps, thereby triggering PriA-mediated overreplication of the termination area, the DnaA-independent growth is triggered by the absence of 3' exonucleases. Overreplication of the termination area in cells lacking 3' exonucleases is abolished if the chromosome is linearized 751755
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.6malfunction the ExoVII- (xseB) and ExoVII- (xseA) deficient mutant strains are both inactive with msDNA. Overexpression of the large subunit is lethal, and cells harboring a plasmid encoding xseA have much lower ExoVII activity than wild-type cells. MsDNA cleavage and cell death at various subunit ratios, overview. The N-terminal domain of XseA causes cell death, and the chromosome is fragmented and condensed in the cells dying by XseA 751292
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.6more under apoptotic conditions, the N-terminal domain of XseA is released from ExoVII through proteolysis by a caspase-like protease and the N-terminal fragment functions in the apoptosis-like cell death of Escherichia coli. ExoVII is composed of two different subunits encoded by xseA (large subunit) and xseB (small subunit) which are both required for catalytic activity 751292
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.6physiological function exonuclease VII (ExoVII) of Escherichia coli is a single strand-specific DNA nuclease, exonuclease VII cleaves msDNA. The biochemical activity of XseA causing cell death is counteracted by the extra amount of the small subunit, XseB. Although the ability to induce cell death is not identical with the single-strand DNA specific nuclease activity, the residues important for nuclease activity are also important for lethality. The N-terminal domain of XseA causes cell death, and the chromosome is fragmented and condensed in the cells dying by XseA. Model for the function of XseA, overview 751292
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.6physiological function inactivation of exonuclease VII by a mutation in its large subunit xseA contributes to attenuation of DNA degradation in UV-irradiated recA mutants. The xseA mutation itself has only a weak effect, however, it acts synergistically with the xonA or sbcD mutations, which inactivate exonuclease I and SbcCD nuclease, respectively, in suppressing reckless DNA degradation. The quadruple xseA xonA sbcD recA mutants show no sign of DNA degradation during post-irradiation incubation, suggesting that ExoVII, together with ExoI and SbcCD, plays a crucial role in regulating RecBCD-catalyzed chromosome degradation. These nucleases may act on double-strand DNA breaks to create blunt DNA ends, the preferred substrates for the RecBCD enzyme. In UV-irradiated recF recA+ cells, the xseA, xonA, and sbcD mutations do not affect RecBCD-mediated DNA repair, suggesting that ExoVII, ExoI and SbcCD nucleases are not essential for the initial targeting of RecBCD to double-strand NA breaks 730437
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.6physiological function mismatch repair proteins MutS and UvrD stimulate single-stranded DNA-specific exonuclease VII action on double-stranded DNA substrates. MutH and MutL proteins show no stimulation of exonuclease VII action. UvrD-mediated stimulation of exonuclease VII action on double-stranded DNA is not accompanied by strand separation. UvrD-mediated stimulation of exonuclease VII action on double-stranded DNA is accentuated by MutS and SSB proteins but not by MutL 707510
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.6physiological function the 3' exonucleases ExoI, ExoVII and SbcCD are involved in termination of DNA. Rinitiated at fork fusion intermediates can result in DNA replication which can sustain cell growth, overview. Levels of overreplication not correlate directly with the ability of cells to grow in the absence of origin firing 751755
Display the word mapDisplay the reaction diagram Show all sequences 3.1.11.6physiological function to stay alive, cells must have either RecG or a 3'-single-stranded DNA exonuclease, which can be exonuclease I, exonuclease VII, or SbcCD. Cells lacking all three nucleases are inviable without RecG -, 715119
Results 1 - 9 of 9