2.7.8.12 | malfunction |
a tagF1-tagF2 deletion mutant of Lactobacillus plantarum, strain WCFS1, lacks poly(glycerol phosphate) polymerase activity required for glycerol-type wall teichoic acid biosynthesis. The mutant activates an alternative genetic locus, tarIJKL, encoding the enzymes for nucleotide activation and incorporation of ribitol in the wall teichoic acid backbone polymer, structure analysis of the alternative ribitol-type wall teichoic acid backbone by HPAEC, UPLC-mass spectrometry, NMR spectroscopy, and MALDI-TOF mass spectrometry, overview, revealing a 1,5-linked poly(ribitol phosphate) which is substituted at the C-2 hydroxyl group of the ribitol residue with alpha-D-glucosyl at a frequency of 28% |
737980 |