EC Number   |
General Information |
Reference |
|---|
   2.7.8.11 | evolution |
the enzyme belongs to the CDP-alcohol phosphatidyltransferase (CAPT) superfamily. Members of the family share the so-called CAPT motif, which forms the active site of all these enzymes. The CAPT motif of phosphatidylinositol synthase Pis1 is located to the cytosolic side, structure comparisons, overview |
-, 737766 |
| 2.7.8.11 | malfunction |
overexpression of the phosphatidylinositol synthase gene (ZmPIS) confers drought stress tolerance by altering membrane lipid composition and increasing abscisic acid synthesis in maize. Lipid profiling analysis shows that, under drought stress conditions, the overexpression of ZmPIS in maize results in significantly elevated levels of most phospholipids and galactolipids in leaves compared with those in wild-type. The expression of some genes involved in the phospholipid metabolism pathway and the abscisic acid biosynthesis pathway including ZmPLC, ZmPLD, ZmDGK1, ZmDGK3, ZmPIP5K9, ZmABA1, ZmNCED, ZmAAO1, ZmAAO2 and ZmSCA1, is markedly upregulated in the overexpression lines after drought stress. The drought stress tolerance of the ZmPIS sense transgenic plants is enhanced significantly at the preflowering stages compared with wild-type maize plants |
-, 739273 |
| 2.7.8.11 | malfunction |
overexpression of ZmPIS leads to elevated levels of phospholipids and galactolipids in transgenic tobacco leaves under drought stress conditions compared to that of wild-type. These changes are correlated with increased drought tolerance |
726251 |
| 2.7.8.11 | malfunction |
removal of the endoplasmic reticulum retrieval sequence from wild-type enzyme Pis1 enhances its activity, suggesting an influence of the lipid environment |
-, 737766 |
| 2.7.8.11 | malfunction |
using a cdipt knockout it shown that cdipt is required for lens structural integrity and photoreceptor cell survival in the zebrafish eye |
724923 |
| 2.7.8.11 | malfunction |
ZmPIS complements the yeast PIS1 mutant phenotype (nonviable on medium with or without lysine) |
726251 |
| 2.7.8.11 | metabolism |
in eukarya, phosphatidylinositol (PI) is only biosynthesized from CDP-diacylglycerol (CDP-DAG) and inositol |
726887 |
| 2.7.8.11 | metabolism |
phosphatidylinositol synthase is a key enzyme in the phospholipid pathway, it catalyzes the first step of the phosphatidylinositol signalling pathway |
-, 739273 |
| 2.7.8.11 | more |
the Pis1 enzyme structure is strongly affected by mild detergents. Modeling of enzyme Pis1 structure, overview |
-, 737766 |
| 2.7.8.11 | physiological function |
the enzyme is responsible for phosphatidylinositol synthesis, which is not only a structural component of cell membranes, but also the precursor of the phospholipid signal molecules that regulate plant response to environment stresses. ZmPIS regulates the plant response to drought stress through altering membrane lipid composition and increasing abscisic acid synthesis in maize |
-, 739273 |
