EC Number   |
General Information |
Reference |
|---|
    2.7.2.11 | malfunction |
the DELTApro1 cells are more sensitive to various stresses than wild-type cells. Yeast cells with gene PRO1 deletion or expressing inactive enzyme display a defect for ribophagy but not for nonselective autophagy. Yeast strains deficient ribophagy are sensitive to nitrogen starvation |
738247 |
| 2.7.2.11 | malfunction |
Yeast cells expressing mutant enzymes D154N and I150T accumulate proline and show a higher tolerance to various stresses, including freezing, ethanol, osmotic pressure, and heat shock |
738715 |
| 2.7.2.11 | metabolism |
gamma-glutamyl kinase is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway |
738247, 738715 |
| 2.7.2.11 | metabolism |
key enzyme in proline biosynthesis |
761649 |
| 2.7.2.11 | more |
conserved residue Asp156 plays an important role in the catalytic activity |
738247 |
| 2.7.2.11 | more |
residues Asp154 and Ile150 in the Saccharomyces cerevisiae gamma-glutamate kinase are important for allosteric regulation and the affinity with L-proline and L-glutamate, but not with ATP. The PUA domain itself is required for the full display of enzyme activity, but is not involved in the allosteric regulation and the affinity with substrates and proline. The proline-auxotrophic yeast strain BY4741DELTApro1DELTAcar2 that expresses the PUA domain deletion-mutant grows even on proline-free medium, although a growth defect is observed |
738715 |
| 2.7.2.11 | physiological function |
Chlamydomonas reinhardtii gamma-glutamte kinase is functional as demonstrated by successful complementation of Escherichia coli auxotroph JW0232 (strain in which the proB gene has been knocked-out) |
722836 |
| 2.7.2.11 | physiological function |
enzyme activation kills Mycobacterium tuberculosis via induction of proline and reactive oxygen species overproduction |
-, 760828 |
| 2.7.2.11 | physiological function |
the enzyme has an alternative function independent of proline biosynthesis. Gene PRO1 genetically interacts with UBP3, which encodes ubiquitin-specific protease, and is required for selective autophagy of ribosomes (ribophagy). The enzyme activity is indispensable for ribophagy, ribophagy is important for cell survival during nitrogen starvation |
738247 |
