EC Number   |
General Information |
Reference |
|---|
    2.3.3.5 | malfunction |
deletion of MCS1 (2-methylcitrate synthase) and MCL1 (2-methylisocitrate lyase) inhibits fungal growth on media containing both glucose and propionate, and media using propionate or propionyl-CoA-producing amino acids (valine, isoleucine, methionine, and threonine) as the sole carbon or nitrogen sources. The DELTAmcs1 mutant forms sparse aerial hyphae and does not produce conidia on complete medium, while the DELTAmcl1 mutant shows decreased conidiation. The aerial mycelium of DELTAmcs1 displays lowered NAD+/NADH ratio, reduced nitric oxide content, and down-regulated transcription of hydrophobin genes. DELTAmcl1 shows reduced appressorium turgor, severely delayed plant penetration, and weakened virulence. Addition of acetate recovers the growth of the wild type and DELTAmcs1 on medium containing both glucose and propionate and recovers the conidiation of both DELTAmcs1 and DELTAmcl1 on complete medium by reducing propionyl-CoA formation. Deletion of MCL1 together with ICL1, an isocitrate lyase gene in the glyoxylate cycle, greatly reduces the virulence of the mutant as compared to the single gene deletion mutants (DELTAicl1 and DELTAmcl1) |
757708 |
| 2.3.3.5 | metabolism |
MCS1 is required for growth and conidiation in Magnaporthe oryzae. Mcs1 is required for propionate utilization |
757708 |
| 2.3.3.5 | metabolism |
methylcitrate cycle |
703115 |
| 2.3.3.5 | metabolism |
the enzyme is essential in propionic acid metabolism |
-, 735795 |
| 2.3.3.5 | metabolism |
the enzyme is involved in the 2-methylcitrate cycle pathway which plays a significant role in the detoxification of propionyl-CoA in bacteria |
757604 |
| 2.3.3.5 | metabolism |
the enzyme is involved in the methylcitric acid cycle |
-, 747131 |
| 2.3.3.5 | metabolism |
the enzyme is involved in the propionate degradation pathway |
755708 |
| 2.3.3.5 | metabolism |
the enzyme is specific to the methylcitrate cycle and essential for mycobacteria to grow on propionate as the sole carbon source |
757102 |
| 2.3.3.5 | metabolism |
the fungi-specific 2-methylcitrate cycle is responsible for detoxifying propionyl-CoA, a toxic metabolite produced as the fungus breaks down proteins and amino acids. The enzyme responsible for this detoxification is 2-methylcitrate synthase (mcsA) and is a potential candidate for the design of new anti-fungals |
756131 |
| 2.3.3.5 | physiological function |
propionyl-CoA detoxification |
705576, 705770 |
