EC Number   |
General Information |
Reference |
|---|
  2.1.2.B2 | evolution |
since MilA have similar mechanism as CH in hydroxyl group formation, it may belong to the superfamily of thymidylate synthase and cytidylate hydroxymethylase |
-, 735967 |
| 2.1.2.B2 | metabolism |
mildiomycin formation pathway catalyzed by MilA and MilB, overview |
-, 735967 |
| 2.1.2.B2 | metabolism |
the enzyme is involved in mildiomycin biosynthesis |
-, 758390 |
| 2.1.2.B2 | more |
six homologous ORFs originally annotated as putative thymidylate synthase are more likely to be CMP hydroxymethylase |
-, 735967 |
| 2.1.2.B2 | physiological function |
several nucleotide-derived nucleoside antibiotics feature modified bases such as hydroxymethyl pyrimidine in polyoxin, and hydroxymethyl cytosine in mildiomycin. The CMP hydroxymethylase named MilA in the biosynthetic pathway of nucleoside mildiomycin in Streptoverticillum rimofaciens ZJU5119 can convert CMP in vitro in the presence of tetrahydrofolate to 5-hydroxymethyl-CMP that is immediately hydrolyzed into free 5-hydroxymethylcytosine by MilB |
-, 735967 |
