EC Number   |
General Information |
Reference |
|---|
   2.1.1.166 | malfunction |
absence of this methylation, which occurs late in the maturation process of the ribosome, appears to cause the destabilization and premature dissociation of the 50 S ribosomal subunit |
704404 |
| 2.1.1.166 | malfunction |
enzyme deletion results in slow growth and accumulation of the 45S particle |
-, 735195 |
| 2.1.1.166 | malfunction |
lack of U2552 methylation, obtained in rrmJ-deficient mutants, results in a decrease in programmed +1 and -1 translational frameshifing and a decrease in readthrough of UAA and UGA stop codons. The increased translational accuracy of rrmJ-deficient strains suggests that the interaction between aminoacyl-tRNA and U2552 is important for selection of the correct tRNA at the ribosomal A site, and supports the idea that translational accuracy in vivo is optimal rather than maximal, thus pointing to the participation of recoding events in the normal cell physiology |
703843 |
| 2.1.1.166 | malfunction |
null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype |
705722 |
| 2.1.1.166 | malfunction |
rrmJ gene deletion causes a severe growth defect and accumulation of aberrant 50S ribosomal subunits in DELTArrmJ. Overexpression of GTPase Der suppresses growth impairment, effects of several Der mutants, overview. In a rrmJ deletion strain, two GTPase domains of Der regulate its association with 50S subunit via the KH-like domain. Phenotypes, overview |
719716 |
| 2.1.1.166 | malfunction |
rrmJ-deficient strain exhibit growth and translational defects compared to the wild-type strain. Growth rates of the rrmJ mutant are decreased at both low and high temperatures. Protein synthesis activity is reduced up to 65% when S30 rrmJ mutant extracts are tested in a coupled in vitro transcription/translation assay. In vitro methylation of these extracts by RrmJ partially restores protein synthesis activity |
702007 |
| 2.1.1.166 | metabolism |
absence of functional RrmJ causes the cellular accumulation of the individual ribosomal subunits at the expense of the functional 70S ribosomes |
704265 |
| 2.1.1.166 | physiological function |
in an Escherichia coli strain lacking SAH nucleosidase Mtn, in which cellular SAM is down-regulated, hypomodification of several methylation sites is observed, including 2'-O-methylation at position 2552 (Um2552) of 23S rRNA. There is a severe growth defect of the strain with significant accumulation of 45S ribosomal precursor harboring 23S rRNA with hypomodified Um2552. The growth defect is partially restored by overexpression of SAM-dependent methyltransferase RlmE |
-, 757859 |
| 2.1.1.166 | physiological function |
the 23S rRNA modification is critical for ribosome stability |
704265 |
| 2.1.1.166 | physiological function |
the enzyme is an assembly factor that acts at a late step of 50S formation |
-, 735195 |
