Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search General Information

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 9 of 9
EC Number General Information Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.4evolution pyruvate formate-lyase activating enzyme (PFL-AE) is a member of the large and diverse radical S-adenosyl-L-methionine (SAM) superfamily, members of which use an iron-sulfur cluster and SAM to initiate difficult radical transformations in all kingdoms of life. Radical SAM enzymes share a common CX3CX2C motif or variation thereof, and the conserved cysteines coordinate three irons of a [4Fe-4S] cluster, while SAM coordinates the fourth iron through its amino and carboxylate moieties 745175
Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.4malfunction mutation of either pflB, which codes for PFL, or pflA, which codes for pyruvate formate lyase activating enzyme, results in abrogation of mixed acid fermentation on galactose, and leads to a decrease in pneumococcal virulence 746477
Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.4metabolism enzyme is activated by pyruvate formate-lyase-activating enzyme by generating a catalytically essential radical on residue Gly734. In the open conformation of the enzyme, the Gly734 residue is located not in its buried position in the enzyme active site but rather in a more solvent-exposed location. The presence of the activating enzyme increases the proportion of enzyme in the open conformation. The activating enzyme accesses residue Gly734 for direct hydrogen atom abstraction by binding to the Gly734 loop in the open conformation, thereby shifting the closed open equilibrium of the enzyme to the right 715533
Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.4metabolism multiple regulators control the transcription of pflA and pflB, some of these regulators are induced by galactose, their control over pflA and pflB is influenced by sodium formate, they exert regulatory influence on each other, and are required for pneumococcal colonization and virulence. Transcriptional profile of mutant DELTApflB compared to the wild-type D39 strain, expression of seven genes annotated as transcriptional regulators is either significantly up or downregulated in the mutant, overview. CcpA, GlnR, and GntR interact with the putative promoters of pflA and pflB, impact of formate on binding affinity of CcpA and GlnR 746477
Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.4physiological function holo-flavodoxin is capable of associating with NADP+-dependent flavodoxin oxidoreductase and pyruvate formate-lyase activating enzyme, whereas there is no detectable interaction between apo-flavodoxin. Holo-flavodoxin interacts with pyruvate formate-lyase activating enzyme with a dissociation constant of 23.3 microM -, 727087
Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.4physiological function PFL-AE is a radical S-adenosyl-L-methionine enzyme that utilizes an iron-sulfur cluster and S-adenosyl-L-methionine to activate pyruvate formate lyase (PFL) via pro-S hydrogen abstraction from Gly734 745316
Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.4physiological function pyruvate formate lyase (PFL), converting pyruvate to formate and acetyl-CoA, is the key enzyme for mixed acid fermentation. Active pyruvate formate lyase (PFL) synthesis is finely tuned, and feedback inhibition and activation involving the pyruvate formate lyase activating enzyme, are detected, analysis of the transcriptional regulation of gene pflA, overview. The pyruvate formate lyase activating enzyme (PFL-AE) is responsible for posttranslational activation of inactive PFL 746477
Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.4physiological function pyruvate formate-lyase activating enzyme (PFL-AE) is a radical S-adenosyl-L-methionine (SAM) enzyme that installs a catalytically essential glycyl radical on pyruvate formate lyase 745175
Display the word mapDisplay the reaction diagram Show all sequences 1.97.1.4physiological function pyruvate formate-lyase-activating enzyme (PFL-AE) activates pyruvate formate-lyase 715533
Results 1 - 9 of 9