EC Number   |
General Information |
Reference |
|---|
  1.5.1.53 | metabolism |
enzyme exhibits ping-pong bi-bi kinetics |
760548 |
| 1.5.1.53 | metabolism |
kinetics suggest a ping-pong mechanism for methylenetetrahydrofolate reductase, or a ternary complex mechanism in which NADPH binding precedes 5,10-methylenetetrahydrofolate |
761412 |
| 1.5.1.53 | metabolism |
Methylenetetrahydrofolate reductase presents reacts with both NADPH and 5,10-methylenetetrahydrofolate at the si face. The reaction involves successive hydride transfers to and from the flavin cofactor. NADP+ dissociates before 5,10-methylenetetrahydrofolate binds the reduced enzyme |
761345 |
| 1.5.1.53 | metabolism |
methylenetetrahydrofolate reductase stereospecifically removes the pro-S hydrogen from the 4-position of NADPH. The oxidation of NADPH is largely or entirely rate limiting in the reductive half-reaction and in NADPH-menadione oxidoreductase turnover at saturating menadione concentration |
760550 |
