EC Number |
General Information |
Reference |
---|
1.2.5.1 | malfunction |
inactivation of the pyruvate:quinone oxidoreductase results in a more efficient L-valine production in Corynebacterium glutamicum strain aceE A16 |
-, 724036 |
1.2.5.1 | malfunction |
there is a positive effect on L-valine production by the inactivation of the pyruvate:quinone oxidoreductase due to increased pyruvate availability |
-, 724069 |
1.2.5.1 | physiological function |
electron transport from pyruvate to oxygen in the liposome system generates a trans-membrane potential of at least 180 mV, negative inside, which is sensitive to the uncouplers carbonyl cyanide p-(trichloromethoxy)phenylhydrazone and valinomycin. A transmembrane potential is also generated by the oxidation of ubiquinol 1 by the terminal oxidase in the absence of the flavoprotein. The pyruvate oxidase can directly reduce ubiquinone 8 within the phospholipid bilayer, menaquinone 8 will not effectively substitute for ubiquinone 8 in this electron-transfer chain, and the cytochrome d terminal oxidase functions as a ubiquinol 8 oxidase and serves as a coupling site in the Escherichia coli aerobic respiratory chain |
702185 |
1.2.5.1 | physiological function |
inactivation of the chromosomal pqo gene leads to the absence of pyruvate:quinone oxidoreductase activity. Growth and amino acid production are not affected under either condition tested. Introduction of plasmid-bound pqo into a pyruvate dehydrogenase complex-negative strain partially relieves the growth phenotype of this mutant, indicating that high pyruvate:quinone oxidoreductase activity can compensate for the function of the pyruvate dehydrogenase complex |
704267 |
1.2.5.1 | physiological function |
the activated enzyme can be efficiently regulated by the oxidation level of the quinone pool in natural membranes |
702204 |
1.2.5.1 | physiological function |
the cytochrome o complex functions as an efficient ubiquinol-8 oxidase in reconstituted proteoliposomes, and ubiquinone-8 serves as an electron carrier from the flavoprotein pyruvate oxidase to the cytochrome complex. Electron flow from the flavoprotein to oxygen in the reconstituted proteoliposomes generates a transmembrane potential of at least 120 mV, negative inside, which is sensitive to ionophore uncouplers and inhibitorso f the terminal oxidase. The minimal composition of this respiratory chain is a flavoprotein dehydrogenase, ubiquinone-8, and the cytochrome o complex |
704373 |