  1.14.13.234 | physiological function |
OxyS catalyzes two sequential hydroxylations at C6 and C5 positions of anhydrotetracycline with opposite stereochemistry. OxyS and reductase OxyR are sufficient to produce the mature tetracycline scaffold, i.e. the complete conversion of anhydrotetracycline to oxytetracycline and tetracycline. OxyS catalyzes the stereospecific hydroxylation of anhydrotetracycline at C-6 (reaction of EC 1.14.13.38). If the released product is captured by EC 1.3.98.4, 5a,11a-dehydrotetracycline dehydrogenase (OxyR), it is reduced to tetracycline. However, if the released product is recaptured by OxyS, it performs an additional hydroxylation at C-5, producing 5a,11a-dehydrooxytetracycline, which, following the action of OxyR, becomes oxytetracycline |
-, 738450 |
