EC Number   |
General Information |
Reference |
|---|
   1.1.1.8 | evolution |
Gpd1p from Saccharomyces kudriavzevii presented five conserved amino acid replacements compared to Saccharomyces cerevisiae (Ala31Ile, Ile67leu, Glu76Asp, Asp142Asn and Ser143Pro) out of 391 total residues, corresponding to an identity of 98.7% |
741300 |
| 1.1.1.8 | evolution |
structural and phylogenetic comparisons reveal four main structure types among the five families of glycerol-3-phosphate and glycerol-1-phosphate dehydrogenases, overview |
721219 |
| 1.1.1.8 | evolution |
the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval |
-, 738723 |
| 1.1.1.8 | malfunction |
Drosophila GPDH-1-null mutants cannot fly |
712632 |
| 1.1.1.8 | malfunction |
mutation N270A results in a 7.7 kcal/mol decrease in the intrinsic phosphodianion binding energy, which is larger than the 5.6 kcal/mol effect of the mutation on the stability of the transition state for reduction of DHAP. A 2.2 kcal/mol stabilization of the transition state for unactivated hydride transfer to the truncated substrate glycolaldehyde, and a change in the effect of phosphite dianion on GPDH-catalyzed reduction of glycolaldehyde, from strongly activating to inhibiting. The N270A mutation breaks the network of hydrogen bonding side chains, Asn270, Thr264, Asn205, Lys204, Asp260, and Lys120, which connect the dianion activation and catalytic sites of GPDH. The disruption dramatically alters the performance of GPDH at these sites |
740076 |
| 1.1.1.8 | metabolism |
glycerol-3-phosphate dehydrogenase (G3PDH) is a key enzyme in the pathway of glycerol synthesis, which converts dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate |
741272 |
| 1.1.1.8 | metabolism |
glycerol-3-phosphate dehydrogenase is a key enzyme in the pathway of glycerol synthesis, which converts dihydroxyacetone phosphate to glycerol-3-phosphate |
-, 723769 |
| 1.1.1.8 | metabolism |
Gpd1p is the flux controlling enzyme in the glycerol biosynthetic pathway |
741300 |
| 1.1.1.8 | metabolism |
isoform G3PD1 plays an important role during fatty acid accumulation in Mortierelle alpine |
-, 740407 |
| 1.1.1.8 | metabolism |
Saccharomyces kudriavzevii has changed the metabolism to promote the branch of the glycolytic pathway involved in glycerol production to adapt to low temperature environments and maintain the NAD+/NADH ratio in alcoholic fermentations. Gpd1p is the flux controlling enzyme in the glycerol biosynthetic pathway |
741300 |
