EC Number   |
General Information |
Reference  |
|---|
   3.4.22.49 | physiological function |
separase plays a pivotal role in the separation of sister chromatids at anaphase by cleaving its substrate cohesin Rad21 |
707105 |
| 3.4.22.49 | malfunction |
loss of separase function during the early mitotic divisions causes cytokinesis failure, depletion of separase causes the accumulation of RAB-11-positive vesicles at the cleavage furrow and midbody |
708239 |
| 3.4.22.49 | physiological function |
separase is required for cytokinesis by regulating the incorporation of RAB-11-positive vesicles into the plasma membrane at the cleavage furrow and midbody |
708239 |
| 3.4.22.49 | malfunction |
loss of separase blocks centriole disengagement during mitotic exit and delays assembly of new centrioles during the following S phase |
708281 |
| 3.4.22.49 | physiological function |
separase acts during M phase to license centrosome duplication |
708281 |
| 3.4.22.49 | malfunction |
Arabidopsis thaliana radially swollen 4 (rsw4), a temperature-sensitive mutant, harbors a mutation in At4g22970, the separase. Loss of separase function in rsw4 at the restrictive temperature is indicated by the widespread failure of replicated chromosomes to disjoin. rsw4 has neither pronounced cell cycle arrest nor anomalous spindle formation, rsw4 roots have disorganized cortical microtubules and accumulate the mitosis-specific cyclin, cyclin B1,1 |
708285 |
| 3.4.22.49 | physiological function |
plant separase, in addition to cleaving cohesin, regulates cyclin B1,1, with profound ramifications for morphogenesis |
708285 |
| 3.4.22.49 | physiological function |
sister chromatid separation at anaphase is triggered by cleavage of the cohesin subunit Scc1, which is mediated by separase |
709217 |
| 3.4.22.49 | malfunction |
meiotic expression of ESP RNA interference blocks the removal of cohesin during both meiosis I and II, results in alterations in nonhomologous centromere association, disrupts the radial microtubule system after telophase II, and affects the proper establishment of nuclear cytoplasmic domains, resulting in the formation of multinucleate microspores |
710317 |
| 3.4.22.49 | physiological function |
Separase is essential for sister chromatid separation during anaphase II. Separase-mediated proteolytic cleavage of the alpha-kleisin subunit of the cohesin complex at the metaphase-to-anaphase transition is essential for the proper segregation of chromosomes. Separase is also involved in mitotic and meiotic anaphase spindle assembly and elongation, interphase spindle pole body positioning, and epithelial cell reorganization |
710317 |
