EC Number   |
General Information |
Reference |
|---|
   3.4.22.49 | malfunction |
Arabidopsis thaliana radially swollen 4 (rsw4), a temperature-sensitive mutant, harbors a mutation in At4g22970, the separase. Loss of separase function in rsw4 at the restrictive temperature is indicated by the widespread failure of replicated chromosomes to disjoin. rsw4 has neither pronounced cell cycle arrest nor anomalous spindle formation, rsw4 roots have disorganized cortical microtubules and accumulate the mitosis-specific cyclin, cyclin B1,1 |
708285 |
| 3.4.22.49 | physiological function |
binding between separase and cyclin B1 is required for the anaphase movement of unpaired sister chromatids. Separase promotes the reversal of Cdk1-mediated phosphorylation on chromosomes at anaphase onset |
731697 |
| 3.4.22.49 | malfunction |
cells depleted of securin or separase display defective acidification of early endosomes and increased membrane recruitment of vacuolar ATPase complexes, mimicking the effect of the specific V-ATPase inhibitor Bafilomycin A1. Securin and separase depletion causes trans-Golgi network and endosome swelling independent of cell cycle. Endosome-mediated receptor degradation and recycling are also significantly impaired by securin and separase depletion, although not receptor internalization or Rab5 activity and autophagy |
718433 |
| 3.4.22.49 | more |
cells expressing wild-type Cdc6 display lower Cdk1 activity and higher separase activity than cells expressing Cdc6 mutant T37V |
718320 |
| 3.4.22.49 | malfunction |
cells that do not express both Cdc55 and securin prematurely separate their sister chromatids, leading to cell death |
718177 |
| 3.4.22.49 | malfunction |
cells that do not express both Cdc55 and securin prematurely separate their sister chromatids, leading to cell death. Mutant mice lacking securin and expressing a non-phosphorylatable separase die in embryonic stage. But mouse embryonic stem cells lacking both these separase regulations can still progress through mitosis in a timely fashion with correct chromosome segregation |
718177 |
| 3.4.22.49 | physiological function |
chiasmata resolution and segregation of homologous chromosome pair. Role in DNA repair. Assembly and elongation of spindle at mitotic anaphase. Spindle formation in meiosis. Karyokinesis (division of nucleus). Spindle midzone assembly. Apoptosis promotion. Cleavage of Slk19. Cdc14 activation and release of Cdc14 from nucleolus |
754237 |
| 3.4.22.49 | physiological function |
chromosomal segregation is mediated by cyclin-dependent kinase 1 and separase, which is regulated by cell division cycle 6, Cdc6, a mitotic substrate of polo-like kinase 1. The phosphorylation of Cdc6 by Plk1 regulates the activity of separase through the association with Cdk1 |
718320 |
| 3.4.22.49 | more |
dynamics of the mitotic exit control system in budding yeast, Queralt's model, modifications, overview. Queralt's model centres around the non-proteolytic function of separase Esp1, which triggers a positive feedback in the activation of MEN by FEAR-induced release of Cdc14 |
718037 |
| 3.4.22.49 | physiological function |
function of separases in metaphase to anaphase transition, overview |
718194 |
