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General Information
RNA MTases from the TrmN/Trm14 family are present in archaea, bacteria and eukaryota and all specifically modify tRNAPhe at guanosine 6 in the tRNA acceptor stem. RNA MTases can be classified into four superfamilies, overview
Trm14 is associated with cluster of orthologous groups, COG, 0116, and most closely resembles the m2G10 tRNA methylase Trm11. Phylogenetic analysis reveals a canonical archaeal/bacterial evolutionary separation with 20-30% sequence identities between the two branches, but it is likely that the detailed functions of COG 0116 enzymes differ between the archaeal and bacterial domains. Phylogenetic distribution of Trm14-like proteins, overview
inactivation of the trmN gene leads to a total absence of N2-methylguanine in tRNA but did not affect cell growth or the formation of other modified nucleosides in tRNA(Phe). Therefore, m2G6 does not appear to be involved in an essential function
physiological function
tRNA m2G6 formation may play a role in stabilizing the structure of the RNA and function synergistically with these core-region modifications to stabilize the molecule against thermal stress. Also, Trm14-catalyzed m2G6 formation in tRNACys may play a role in modulating the aminoacylation efficiency of phosphoseryl-tRNA synthetase, i.e. SepRS. Control of SepRS (or CysRS) activity by SAM-dependent methylation of tRNACys might provide the methanogen cell with a regulatory mechanism by which protein synthesis rates can respond to environmental sulfur levels or to concentrations of sulfur metabolites
Results 1 - 4 of 4